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1. Repeating these steps produced a plasmid containing two protein A domains, and then by another step to one with three domains.

2. Plasmid: circular DNA molecules able to replicate independently of the chromosome in microorganisms.

3. However, the genes brought in by a plasmid do not usually become a permanent part of the bacterium's own chromosome.

4. However, as described above, this plasmid was found to exhibit wild type R-M.

5. Chickens were immunized intramuscularly with the naked DNA plasmid.

6. BVTech Plasmid is DNA plasmid drawing software free software.

7. Figure 2 . Restriction pattern of the recombinant plasmid.

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8. Objective Construct a recombinant plasmid pET 28 a - EDA - EDB , prepare the fusion EDA - EDB protein.

9. Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.

10. What will happen to the mobility of the plasmid as you add increasing amounts of ethidium bromide to the gel during electrophoresis?

11. The preparation process of the immunotoxin plasmid includes the construction of the recombinant plasmid, the preparation of the recombinant transinfected engineering bacterium and other steps.

12. The recombinant bait plasmid didn't have self-activating effect and did not show toxicity to yeast AH109 cell.

13. Objective To construct recombinant plasmid for expression of chymopapain in E.

14. Objective: Study on extraction of plasmid DNA with phenol - chloroform from clone.

15. Results: The recombinant plasmid pUC 18 E 6 had been got.

16. Each plasmid contains a cDNA insert that was cloned via EcoRI and XhoI.

17. Curing the R plasmids from E. coli J53, the activity of L-asparaginase increased and was close to that of the plasmid free strain.

18. The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn.

19. This provides strong evidence that the hybridization signals obtained are highly specific and due to the transfected plasmid.

20. The predicted 5' and 3' boundaries of the expression regions were confirmed by direct plasmid DNA sequencing.

21. We therefore took this data into consideration when calculating the superhelical densities of the different plasmid preparations.

22. T3 symbolizes the T3 RNA polymerase promoter of a bluescript plasmid.

23. The sequences at the 5' and 3' ends of the insertion were confirmed by direct plasmid sequencing.

24. After extracting with phenol-chloroform, it is not necessary to recover the plasmid by ethanol precipitation.

25. PC - 3 cells were transfected with mGM - CSF expressing plasmid or the control vector by lipo - some transfection.

26. Objective To select an optimal combined set of carrier, introducing route and dose of recombinant plasmid of augmenter of liver regeneration(ALR) for ALR gene therapy.

27. We cloned CaGUA1 gene into the vector pCR4, a expression plasmid vector used in C. albicans. We transformed this recombinant clone into the deletion mutant of CaGUA1.

28. The ability of amphiphilic block copolymers that consist of polyethylenimine (PEI) and poly(L-lactide) (PLLA) to modulate the delivery of plasmid DNA was evaluated.

29. The constitutive expression vector of the present invention is inducible plasmid vector with regulating gene eliminated or deleted.

30. In this review, we summarize the current progresses on plasmid biology in the rare actinomycetes.