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1) AIM: To establish an immortalized human odontoblast cell line.

2) AIM:To cultivate primary human odontoblast.

3) Objective: To observe the relationship between the odontoblast process and the dentinal tubules.

4) The expression of odontoblast significantly decreased in the late bell stage,(http://sentencedict.com/odontoblast.html) while ameloblast showed positive expression.

5) In the cap stage, odontoblast, ameloblast and the adjacent dental papilla expressed the OSX protein strongly. OSX protein also existed in enamel-dentinal junction, the new enamel and dentinal tubule.

6) The results of this study have provided theoretic basis for further investigation on the mechanism of the calcium transportation, dentin mineralization and signal transduction hi odontoblast cells.

7) RA legion was presented because water and protein lost. )That amelogenin spread into dentin could promote the odontoblast differentiation and induce dentin calcification.

8) L-type calcium channel played a very important role in intracellular calcium transportation of odontoblast cells and involved in the process of the dentin mineralization.

9) Conclusion:FGF18 may play a role during the development of dental germ and could improve the proliferation and differentiation of odontoblast.

10) An ideal pulp capping agent should have properties to induce odontoblast differentiation and reparative dentinogenesis.

11) RESULTS: In early age of tooth development, MEPE was expressed in ameloblast, odontoblast and pulp tissue.

12) Dentin matrix protein 1 (DMP1) is expressed in both pulp and odontoblast cells and deletion of the Dmp1 gene leads to defects in odontogenesis and mineralization.

13) BMP was expressed in the ameloblast, the intermedium stratum cell and the stellate reticulum cell but not in dental papillae cells or odontoblast.

14) In the previous researches, it is always demanded to separate the differentiated stages of DPSC which are always identified by the molecular markers in odontoblast.